Surface immobilizable chelator for label-free electrical detection of pyrophosphate
نویسندگان
چکیده
منابع مشابه
Surface immobilizable chelator for label-free electrical detection of pyrophosphate.
A new pyrophosphate (PPi) chelator was designed for surface-sensitive electrical detection of biomolecular reactions. This article describes the synthesis of the PPi-selective receptor, its surface immobilization and application to label-free electrical detection on a silicon-based field-effect transistor (FET) sensor.
متن کاملSurface immobilizable chelator for label-free electrical detection of pyrophosphatew
Nucleotide incorporation reactions, catalyzed by DNA and RNA polymerases, are critically important in the biological processes of living systems. Their common byproduct, pyrophosphate (PPi), is a negatively charged small molecule typically detected using optical techniques, such as chemiluminescence. Label-free electrical monitoring of biochemical reactions offers several advantages including i...
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متن کاملLabel-free electrical detection of pyrophosphate generated from DNA polymerase reactions on field-effect devices.
We introduce a label-free approach for sensing polymerase reactions on deoxyribonucleic acid (DNA) using a chelator-modified silicon-on-insulator field-effect transistor (SOI-FET) that exhibits selective and reversible electrical response to pyrophosphate anions. The chemical modification of the sensor surface was designed to include rolling-circle amplification (RCA) DNA colonies for locally e...
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ژورنال
عنوان ژورنال: Chemical Communications
سال: 2011
ISSN: 1359-7345,1364-548X
DOI: 10.1039/c1cc12073e